efficacy of dna typing as an accurate method in forensic medicine

نویسندگان

حسن توفیقی

tofighi h حسین حسنیان مقدم

hassanian moghaddam h معصومه ناجی

naji m افروز نیکبخت دهکردی

nikbakht dehkordi a هادی نمازی

چکیده

dna typing is a new method with important applications in forensic medicine. in the present study, we evaluated application of dna typing in iran. loci hum lpl, hum tpox, hum f13, hum vw 31a, hum th01 and hum fes/fps of dna short tandem repeats were studied. to determine sensitivity of the test, 85 mother-child couples (1020 chromosomes) that were referred to dna section of legal medicine organization of iran were included and for determination of it's specificity 42 brother-sister couples (1200 chromosomes) and 58 non-relative couples were examined. the results show lack of mutations in the studied loci and acceptable sensitivity of the test. of 12 alleles of siblings, there were 2-6 differences, in contrast with 3-9 differences in non-relatives, so the test has 100% specificity in these loci. considering polymorphism, power of exclusion of these 6 sites was 99%.

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عنوان ژورنال:
مجله دانشکده پزشکی دانشگاه علوم پزشکی تهران

جلد ۵۸، شماره ۳، صفحات ۴۲-۴۵

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